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Elevage Informations à propos de l’élevage, portées...

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Old 28-08-2008, 10:54   #1
elf
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Quote:
Originally Posted by elf
Quote:
Ce ne sont pas des marqueurs de race : ils ne
permettront pas de déterminer si les parents de Vorss sont des loups à
100 % ou des hybrides.......

Comme je suis une personne obstinée, je vais prendre contact avec le
Service de Recherche et Développement, pour demander si l'on peut
obtenir d'autres informations complémentaires par un biais
différent.....
I was working for a while on DNA microsatellite evolution and I'm
pretty sure you can check whatever you want but it's time and money ,
except if it fits on a R&D program. I guess I remember that the
"Laboratoire d'Ecologie Alpine de Grenoble" was doing research on
wolves DNA at some point, worth checking there if you need deeper
tests one day.
Regards.
Quote:
Originally Posted by elf
Quote:
Originally Posted by michaelundinaeichhorn
As far as I know this can only be done by one labaratory in Poland and
not as easily as you should think.

Ina
Those methods are well known among population genetics researchers,
the fact that they are mostly not publicly available is another story.
With allelic/haplotypic frequencies of sub-species (Italian, American
etc.) one can determine the population origin of an individual. In
addition DNA microsatellites evolutionary models are more and more
accurate and some suitable markers are available, so we can also have
an estimation of the divergence degree.
The fact that these methods are mostly not publicly available is owing
to time and money, for example I had the confirmation that the
laboratory I told about can provide these methods (they won't because
have not the time for that), also they are not astonished that
"Antagène" (the French laboratory that provide DNA tests) does not
provide it.
Quote:
Originally Posted by Lorry - MLS
Quote:
Originally Posted by elf
I was working for a while on DNA microsatellite evolution and I'm
pretty sure you can check whatever you want but it's time and money ,
except if it fits on a R&D program. I guess I remember that the
"Laboratoire d'Ecologie Alpine de Grenoble" was doing research on
wolves DNA at some point, worth checking there if you need deeper
tests one day.
Regards.
Hello,
Let us can continue this conversation on the French Forum ?
I not mastery not enough English writes to answer you and I am not
admitted (supposed) on the English Forum, if I write in Frenchman

Thank you in advance....
Lorry Leclerc
Quote:
Originally Posted by saschia
Quote:
Originally Posted by elf
TIn addition DNA microsatellites evolutionary models are more and more
accurate and some suitable markers are available, so we can also have
an estimation of the divergence degree.
Yes, but this works (as far as I know) for populations diverged for
some significant time. As all CSW are actually descendants of one
female wolf, and several female GSD, and as the first pairing was done
some 50 years ago, you would get results that some percent of CSW
population are 100% GSD and other percent of CSW are 100% carpathian
wolf, and the percentage of GSD or wolf in the population of CSW would
depend only on the relative amount of female lines used, so in theory
if only male descendants of the wolf Brita were used, than according
to mitochondria only GSD are present, or on the other hand, if only
male descendants of the GSD females were used, than we have only wolf
mitochondria...
Of course assuming that actually mitochondria from sperm do not play role.

Does anybody know, if there was found any significant difference in
overall DNA between wolf and dogs other than genes for skin/coat/eye
pigmentation?
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Saschia
(Sasa Zahradnikova)

Quote:
Originally Posted by elf
Quote:
Yes, but this works (as far as I know) for populations diverged for
some significant time. As all CSW are actually descendants of one
female wolf, and several female GSD, and as the first pairing was done
some 50 years ago, you would get results that some percent of CSW
population are 100% GSD and other percent of CSW are 100% carpathian
wolf, and the percentage of GSD or wolf in the population of CSW would
depend only on the relative amount of female lines used, so in theory
if only male descendants of the wolf Brita were used, than according
to mitochondria only GSD are present, or on the other hand, if only
male descendants of the GSD females were used, than we have only wolf
mitochondria...
Of course assuming that actually mitochondria from sperm do not play
role.
To get ride of sex biaised inheritance mtDNA is used in combination
with nuclear Y-chromosome and autosomal markers. Furthermore
microsatellites are VERY more polymorphic than any other makers, this
solves the "signifiant time" needed.

Quote:
Does anybody know, if there was found any significant difference in
overall DNA between wolf and dogs other than genes for skin/coat/eye
pigmentation?
I guess this is not the way to look for, promising ways are
modifications of mRNA expression and genetic regulatory networks.
Quote:
Originally Posted by Margo
Slovakian Club decided that the dog writen on register in Italy (LIR), France ("titre initial"), Germany (VDH R) or in any other country are considered to be not PUREBREED Czechoslovakian Wolfdogs.

Ne serait-t-il pas plus intelligent que les clubs d'origines mettent un peu d'argent dans la recherche de tests spécifiques à la race plutôt que de réduire encore plus le pool génétique ...
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Old 28-08-2008, 10:57   #2
shakespeare
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Ah ben oui bien sûr, certes, bonne idée, mais comme la plupart des êtres humains, il est difficile d'accepter de sortir de l'argent et investir pour que ça paie ensuite (en argent ou en qualité) plutôt que d'encaisser tout de suite. C'est un paradoxe étrange d'associer des êtres vivants à l'argent, ça conduit à de drôles de choses et des égarements déontologiques parfois...
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