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Old 09-04-2009, 13:52   #1
mijke
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Default Pituitary Dwarfism test for CsW is available!

The university of Utrecht did validate the pituitary dwarfism test for the CsW breed

And since April 2009 it also possible to test a CsW for pituitary dwarfism!

Unfortunately also in CsW breed several dwarfs (from several lines) are known.

And when 1% is dwarf in a population, 18 % will be carrier of this mutated gen!
And the number of carriers in practice is often much higher than thought




But as of now there is no need that more CsW dwarfs will be born!

Because of a simple test can be established if a dog is carrier of this mutated gene.

And not any dog has to be out of breeding program when he/she is carrier.
The only thing is not to make any crossings between carrier x carrier!(of this mutated gen)


Therefore it makes sense to test as much as possible CsW's to see how this mutation in the breed is spread.
And to prevent another CsW dwarf will be ever born, you have to test all CsW's that will be used for breeding!




How an owner can test his CsW for pituitary dwarfism:

Because not all vets will know the test, it can be useful to give them a copy of the article of dr. H. Kooistra en drs. A. Voorbij: Pituitary dwarfism in German shepherd dogs and Saarloos wolfhounds -Availability of a genetic test-
Here you can download this document as pdf file **


A form blood sample is here to download as pdf file ** .

** A pdf file is to open with program Acrobat Reader. You can download this program for free for example Here
  • The owner can fill in all the info about himself and the dog in the form: ”Blood DNA research Pituitary Dwarfism” and sign it.
  • Then the owner can go to his own veterinarian with his CsW, the form the pedigree and a copy of the pedigree.
  • The vet has to check the chip number, fill in the info and has to sign the form.
  • Then he can collect 4ml EDTA blood sample.
The 4 ml blood sample (collected in an EDTA containing tube) can together with the signed form and the copy of the pedigree send by the veterinarian to:

Dr. H.S. Kooistra
Department of Clinical Sciences of Companion Animals
Faculty of Veterinary Medicine, Utrecht University
Yalelaan 108
3584 CM Utrecht
The Netherlands



  • After the test the university will send a bill to the owner
  • The costs of this genetic test is Euro 100 (excl. VAT).
  • When the university has received the paying of the owner, they will send him the test result.
  • When the dog is free of dwarfism gen, the owner also receives an official certificate.

Here some general info about pituitary dwarfism:



What is pituitary dwarfism:
Dwarfism is a horrible incurable disorder which you don’t wish to any dog. A dwarf has a badly developed pituitary , which means that certain hormones (such as growth hormones) are not produced. Due to the shortage of the production of the thyroid stimulating hormone, they have a slow functioning thyroid. Besides the fact that these dogs stay small, they are also haunted by various horrible side effects (e.g. baldness, itching, inflammations, malfunctioning of the liver and kidneys, slow behaviour) when they are not treated with medicines on a daily basis.



Recognising and existence of dwarfism in a breed:
Dwarfism is not always recognised by a breeder!!
Many dwarfs die in the uterus or die during the birth.
And moreover 90% of the living born dwarfs die in the first week.
Pups behind in growth are often not recognised as dwarfs. After (4 weeks or later) people see that a pup is different in comparison with the other pups. Dwarfism is known in the breeds German Shepherd, Karelische Bearsdog, Saarlooswolfhond and the Czechoslovakian Wolfdog.

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Old 09-04-2009, 14:08   #2
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The CsV breed and future puppies thanks you for your EXCELLENT WORK !!!

Very best regards / Mikael & Hronec
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Old 09-04-2009, 14:34   #3
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Well done Mijke.
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Old 09-04-2009, 15:36   #4
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Thanks a lot for your effort Mijke

I will test both of my dogs soon...

Greetings Rolf
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Old 09-05-2009, 23:06   #5
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Last update May 2009:

8 CsW’s were already tested (7 from Holland and 1 of Belgium)

From these CsW’s are 6 free of this gen mutation.

And 2 are carrier of dwarfism gen. One of them is from Dutch breeding line and the other was imported from CZ.

When I have received the copy’s of this results, I’ll publish all the info (with names of the dogs)
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Old 10-05-2009, 03:29   #6
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Scary...

But very good that there is a test now...Thanks for all of the work and updates, Mijke...

Marcy
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Old 09-06-2011, 23:14   #7
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Hello,

To anyone that has had this test done, I was wondering something..

Does the blood sample has to be spun and separated from the clot? We did the rabies antibody test before and this was the procedure, but I am not sure if it is the same for also this test.

Also, the sample must be sent express overnight, correct?

Thank you.
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Old 09-06-2011, 23:32   #8
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You must sent EDTA blood for DNA testing , no centrifugation!!!

Try to get the blood to the lab as fresh as possible, a copple of day's won't be a problem
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Old 09-06-2011, 23:36   #9
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Quote:
Originally Posted by jmvdwiel View Post
You must sent EDTA blood for DNA testing , no centrifugation!!!

Try to get the blood to the lab as fresh as possible, a copple of day's won't be a problem
I forgot to add that I do not know what "EDTA" is, but I read something now and I understand it to be some sort of anticoagulant that is already in the tube.

Thank you!!!
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Old 10-06-2011, 00:17   #10
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Quote:
Originally Posted by yukidomari View Post
I forgot to add that I do not know what "EDTA" is, but I read something now and I understand it to be some sort of anticoagulant that is already in the tube.

Thank you!!!
Purple top tube. The sample I sent for DM to Missouri had to be placed on a (heavy) chillpack, and then insulated according to postal regulation in a box inside a box (one had to be leakproof). I had to send 5 tubes, but I think that was part of the study UofM was doing. It cost me $65 to send the blood from Virginia to Missouri overnight. I think my vet charged me like $15-20 for the blood draw. Vets will often have little insulated boxes and freeze packs that they get with meds that they will give you. I'm not sure if there are any customs regulations related to exporting blood from the US? Laboklin offers a buccal (saliva) test, but it costs more (all testing still done in Utrecht). Maybe it's possible to send these swabs directly to Utrecht instead?
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Old 10-06-2011, 00:41   #11
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Quote:
Originally Posted by GalomyOak View Post
I'm not sure if there are any customs regulations related to exporting blood from the US? Laboklin offers a buccal (saliva) test, but it costs more (all testing still done in Utrecht). Maybe it's possible to send these swabs directly to Utrecht instead?
I found this: http://www.biofocus.de/PDF/Onkologie...e_Handling.pdf

It's for human blood sampling but I assume shipping-wise largely the same? Anyway, when have the test done I will call FedEx and confirm. Thanks for the heads up though. I hadn't thought about that yet. Does anyone know if the blood samples to Utrecht also have to be chilled?
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