Hi, I am doing progesteron tests right now with Frei, as we should travel quite far, so I hope it will help. Of course I am also watching her behavior and it seems to correlate.
I was just telling my friend that I would really like some research on hormonal changes in bitches, meaning day-to day changes, oscillation (Frei went from 1.72 to 1.15 in 3 days and then to 2.39 in another 3) etc. But that would require some research grant (having test every 3rd day is quite a lot for me), otherwise it is pretty fast, takes less than 2 hours.
I found a paper where they have studied captive wolves (both kenneled and roaming) and measured their progress through estrus, but they had quite a long spacing between measurement of the same bitch due to stress probably. But they found out that proestrus is cca 2x as long as in dogs, while estrus and gravidity is the same and also hormonal levels are the same.
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